距离上一次猫咪和狗狗的大战已经过去了数年之久，来之不易的和平再一次受到威胁。这次的敌人可不是上一部中的笨蛋，它可是原猫咪间谍组织“喵” （MEOWS）的顶级特工，名为Kitty Galore。它顺利地窃取到了狗狗组织的核心机密，并且为了报复狗狗组织和人类，它设计了一个恐怖而毒辣的计划意图控制全世界！在狗狗联合国的一致授意 下，狗类和猫咪破天荒地结为战略同盟，帮助主角走上阻挠邪恶计划的征途......

In the age-old battle between cats and dogs, one crazed feline has taken things a paw too far.

Kitty Galore, formerly an agent for cat spy organization MEOWS, has gone rogue and hatched a diabolical plan to not only bring her canine enemies to heel, but take down her former kitty comrades and make the world her scratching post.  Faced with this unprecedented threat, cats and dogs will be forced to join forces for the first time in history in an unlikely alliance to save themselves—and their humans—in “Cats & Dogs: The Revenge of Kitty Galore,” a comedy in 3D that blends live action with state-of-the-art puppetry and computer animation.

 It's time for the fur to fly.